Lactose metabolism by Streptococcus mutans: evidence for induction of the tagatose 6-phosphate pathway.
نویسندگان
چکیده
Growth on lactose by strains of Streptococcus mutans resulted in the induction of the lactose-phosphoenolpyruvate-phosphotransferase system, phospho-beta-galactosidase, and the enzymes of the tagatose 6-phosphate pathway.
منابع مشابه
Isolation, characterization and nucleotide sequence of the Streptococcus mutans lactose-specific enzyme II (lacE) gene of the PTS and the phospho-beta-galactosidase (lacG) gene.
The lacE and lacG genes from Streptococcus mutans have been isolated and characterized, and their nucleotide sequence has been determined. The lacE gene encodes the lactose-specific Enzyme II component of the phosphoenolpyruvate-dependent phosphotransferase system (PTS). The lacG gene encodes the phospho-beta-galactosidase which cleaves the lactose phosphate that is formed by the lactose PTS. T...
متن کاملStreptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster.
DNA cloned into Escherichia coli K-12 from a serotype c strain of Streptococcus mutans encodes three enzyme activities for galactose utilization via the tagatose 6-phosphate pathway: galactose 6-phosphate isomerase, tagatose 6-phosphate kinase, and tagatose-1,6-bisphosphate aldolase. The genes coding for the tagatose 6-phosphate pathway were located on a 3.28-kb HindIII DNA fragment. Analysis o...
متن کاملClustered genes for galactose metabolism from Streptococcus mutans cloned in Escherichia coli.
DNA cloned into Escherichia coli from a serotype c strain of Streptococcus mutans allowed a galKTE mutant to utilize galactose for growth. However, the DNA does not appear to encode enzymes of the Leloir pathway used by E. coli, but rather appears to encode enzymes of the tagatose phosphate pathway.
متن کاملLactobacillus casei 64H contains a phosphoenolpyruvate-dependent phosphotransferase system for uptake of galactose, as confirmed by analysis of ptsH and different gal mutants.
Galactose metabolism in Lactobacillus casei 64H was analyzed by genetic and biochemical methods. Mutants with defects in ptsH, galK, or the tagatose 6-phosphate pathway were isolated either by positive selection using 2-deoxyglucose or 2-deoxygalactose or by an enrichment procedure with streptozotocin. ptsH mutations abolish growth on lactose, cellobiose, N-acetylglucosamine, mannose, fructose,...
متن کاملD-Tagatose 6-Phosphate Pathways1
Lactose is usually metabolized by A-galactosidase-catalyzed cleavage to D-glucose and D-galactose, followed by the conversion of Dgalactose to D-glucose 6-phosphate via the well known Leloir pathway (10): D-galactose *D galactose 1-phosphate D-glucose 1-phosphate D-glucose 6-phosphate. In Staphylococcus aureus, however, the disaccharide is utilized exclusively via lactose phosphate, which is cl...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 140 3 شماره
صفحات -
تاریخ انتشار 1979